Role and Mechanism of Chronic Restraint Stress in Regulating Energy Metabolism and Reproductive Function Through Hypothalamic Kisspeptin Neurons

Abstract Objectives: The purpose of this study was to investigate whether stress affected energy metabolism and reproductive function through the glucocorticoid receptor (GR) on Kisspeptin neurons in hypothalamus. Methods: 1. first part focused effects chronic restraint mice. 2. second expression serum cortisol prolactin GR Kiss1 genes hypothalamus.3. Based above research, we constructed specific knock out (KGRKO) mice were subjected intervention, body weight monitored every day, vaginal smear performed female observe estrous cycle. After 28 days stress, collected detect sex hormone levels, including LH, FSH estrogen levels testosterone level male by ELISA. Results: 1.The intake group lower than those control. 2.After showed irregular LH significantly decreased compared with control group.3. higher group, hypothalamus gene mRNA protein decreased.4. In mice, GRflox/flox/Kiss1Cre-control nadir increased morning GRflox/flox/Kiss1Cre+ KGRKO however, there no significant difference peak afternoon. Compared + had a loss group. KGRKO+restraint reduced loss, suggesting that might partially affect neurons. Conclusions: Chronic induced reversed gain high-fat diet. played negative role regulating function. reproduction mediated This provided central mechanism for affecting function, great significance revealing relationship between HPA axis reproduction.